Application | Comment | Organism |
---|---|---|
molecular biology | the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences and hence are important functional genomics tool for tagging and cloning of unknown gene sequences | Zea mays |
Cloned (Comment) | Organism |
---|---|
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta2 (DE3) in soluble form, end-point RT-PCR confirms the integrity of AcTPase mRNA during cell culture, RT-PCR expression analysis | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Zea mays | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
90000 | - |
- |
Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | P08770 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged enzyme from Escherichia coli strain Rosetta2 (DE3) by nickel affinity chromatography | Zea mays |
Subunits | Comment | Organism |
---|---|---|
? | x * 90000, about, recombinant full-length enzyme, SDS-PAGE | Zea mays |
More | AcTPase is an 807aa protein and consists of three N-terminal nuclear localization signals (residues 44-206), a bipartite DNA binding domain (159-206), a catalytic core domain, and a highly conserved C-terminal dimerization domain (674-754). The catalytic core domain of AcTPase is thought to form a retroviral integrase-like fold | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
Ac transposase | - |
Zea mays |
AcTPase | - |
Zea mays |
maize activator transposase | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the family of hAT transposases | Zea mays |
physiological function | the maize activator (Ac) transposase recognizes and excises Ac and Dissociation (Ds) elements and mediates insertion elsewhere in the genome. Insertions of Ds can cause disruption in gene sequences, involvement of Ac transposase in Ds movement | Zea mays |